What applications is this CD27 antibody validated for?

This Recombinant Human antibody (MOB-096LC) is validated for use in Enzyme-linked Immunosorbent Assay (ELISA), Functional Studies (FuncS), and Western Blot (WB). It specifically recognizes human CD27, a member of the TNF-receptor superfamily.

How is this antibody purified?

The antibody is purified by affinity chromatography to ensure high purity (>95% by SDS-PAGE). It is a Human IgG1, kappa antibody expressed in mammalian cells, ensuring consistent quality for your research into T cell immunity.

Human Anti-CD27 Recombinant Antibody (MOB-096LC)

CAT#: MOB-096LC

This product is a recombinant human antibody that recognizes human CD27. The antibody was purified by affinity chromatography.

Tested Data Gene Expression

ELISA

Figure 1 Anti-CD27 Recombinant Antibody (MOB-096LC) in ELISA

ELISA analysis of MOB-096LC was performed by coating with Human PCNA Protein (His Tag). Then blocked with BSA and incubated with MOB-096LC. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-CD27 Recombinant Antibody (MOB-096LC) in WB

Western blot analysis of MOB-096LC was performed with Human CD27 Protein (His Tag) in reduced condition Lane 2 and Lane 3 (0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with MOB-096LC (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 3 Anti-CD27 Recombinant Antibody (MOB-096LC) in DB

Dot Blot analysis of MOB-096LC was performed by coating with Human CD27 Protein (His Tag).

MOB-096LC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control, PC: Positive control

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Normal Tissue Figure 1 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_8_8.jpg

Normal Tissue Figure 2 Tonsil

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2420/6097_A_5_8.jpg

Normal Tissue Figure 3 High expression in lymph node.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_7_8_val_selected.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_8_8.jpg

Normal Tissue Figure 5 Tonsil

Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2420/6097_A_5_8.jpg

RNA Expression Figure 3 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000139193-CD27

RNA Expression Figure 6 RNA cell line category: Group enriched (Daudi, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139193-CD27

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Specifications

Immunogen
hCD27-Fc fusion protein

Host Species
Human

Type
Human IgG1, κ

Specificity
Human CD27

Species Reactivity
Human

Applications
ELISA, FuncS, WB

Related Disease
Lymphoma

Product Property

Purity
>95% as determined by SDS-PAGE

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
CD27; CD27 molecule; T14; S152; Tp55; TNFRSF7; S152. LPFS2

Gene ID
939

UniProt ID
P26842

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Breast cancer biomarkers at key points during disease progression

High Affinity For CD27

This recombinant human antibody demonstrated strong binding to human CD27 in our ELISA setups. The affinity chromatography purification resulted in a highly pure product (>95%) that gave us consistent readings. It has been instrumental in our studies of T cell immunity and B-cell activation regulation.

Effective in Functional Assays

We used MOB-096LC in functional studies to explore CD27 signaling. It performed reliably as a Human IgG1 kappa antibody. Its ability to interact with the target receptor allowed us to model downstream signaling events like NF-kappaB activation in our in vitro cellular systems.

Q&As

What applications is this CD27 antibody validated for?

A: This Recombinant Human antibody (MOB-096LC) is validated for use in Enzyme-linked Immunosorbent Assay (ELISA), Functional Studies (FuncS), and Western Blot (WB). It specifically recognizes human CD27, a member of the TNF-receptor superfamily.
How is this antibody purified?

A: The antibody is purified by affinity chromatography to ensure high purity (>95% by SDS-PAGE). It is a Human IgG1, kappa antibody expressed in mammalian cells, ensuring consistent quality for your research into T cell immunity.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-096LC.pdf

MSDS

MSDS - MOB-096LC.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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